HIF1A Knockout cell line (THP-1)

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HIF1A Knockout cell line (THP-1) Order

HIF1A Knockout cell line (THP-1)
Catalog#
YKO-HT0027
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HIF1A Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HIF1A Knockout cell line (THP-1)
Species
Human
Gene Name
HIF1A
Gene ID
3091
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript HIF1A-202)
Validation: PCR+Sanger Sequencing
HIF1A Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HIF1A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HIF1A gene knockout HIF1A Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
HIF1A Knockout cell line (THP-1)
HIF1A Knockout cell line (THP-1)
Catalog#
YKO-HT0027
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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